The works in my group mainly focus on developing different reaction-based probes or nanomaterial probes and their application in cell imaging. We have developed a series of subcellular-organelle-targeted probes for imaging and detection of reactive oxygen species (ROS), reactive nitrogen species (RNS) and reactive sulfur species (RSS) in living cells. Meanwhile, we have designed purine-based AIEgens which exhibit lipid droplets specific property in live cellular imaging; based on this skeleton, a wash-free imaging and ultrafast staining process for plasma membrane was developed with good biocompatibility. Currently, we are developing near-infrared (NIR) fluorescent dyes with large stokes shift, including phosphorus-substituted rhodamine and sulfoxide-substituted rhodamine, and investing their application in autophagy and fluorescence-guided surgery. We also focus on selective distinguish and detection of modified bases in DNA methylation. By exploring reaction-based labelling/fluorescent reagents, we have realized selective detection of 5fC and 5fU, respectively. We are developing a photodissociation technique for selective enrichment of modified bases and single base resolution sequencing.
Education And Academic Experience：
2016.9-Present：Professor, College of Chemistry, Sichuan University
2011.7-2016.8：Associate Professor, College of Chemistry, Sichuan University
2010.11-2011.10: Postdoctoral Fellow, The University of Hong Kong
2008.7-2011.6：Lecture of Sichuan University
2003.9-2008.6: Ph.D., Sichuan University
1999.9-2003.7：B.S., Yantai University
Honors and Awards：
The 6th China International College Students’ ”Internet+” Innovation and Entrepreneurship Competition Silver award advisor （2 nd）
Reserve candidates of academic and technical leaders of Sichuan province
Editorial/Advisory Board Memberships：
《Chinese Chemical Letters》 Youth Editorial Board Member
《Current Chinese Chemistry》 Editorial Board Member
《Chinese Journal of Analysis Laboratory》 Youth Editorial Board Member
Publications:
1、Shi, L.; Liu, Y.-H.; Li, K.*; Amit, S.; Yu, K.-K; Ji, M. S.; Li, L.-L.; Zhou, Q.; Zhang, H.; Kim，J. S.*; Yu, X.-Q.* Rapid and ultrasensitive imaging of plasma membrane with AIE based probe in bio-systems Angew. Chem. Int. Ed. 2020, 59, 9962-9966.
2、Lu, P.-L.; Li, K.*; Shi, L.; Liu, X.; Feng, M.-L.; He, H.-Z.; Yang, H.; Yu, X.-Q.* Donor and acceptor engineering for BINOL based AIEgens with enhanced fluorescence performance. Mater. Adv. 2020, 1, 61-70.
3、Zhou, Q.; Li, K.*; Yu, K.-K.; Li, N.; Shi, L.; Chen, H; Chen, S.-Y.; Yu X.-Q.* Aqueous Wittig Reaction-mediated Fast Fluorogenic Identification and Single-base Resolution Analysis of 5-Formylcytosine in DNA. Chem. Commun. 2020, 56, 12158-12161
4、Shi, L.; Li, K.*; Liu, Y.-H.; Liu, X.; Zhou, Q.; Chen, S.-Y.; Yu, X.-Q.* Bio-inspired assembly in a phospholipid bilayer: effective regulation of electrostatic and hydrophobic interactions for plasma membrane specific probes. Chem. Commun. 2020, 56, 3661-3664.
5、Li, M.-Y.; Li, K.*; Liu, Y.-H.; Zhang, H.; Yu, K.-K; Liu, X.; Yu, X.-Q.* Mitochondria- Immobilized Fluorescent Probe for the Detection of Hypochlorite in Living Cells, Tissues, and Zebrafishes Anal. Chem. 2020, 92, 3262-3269.
6、Wang, N.; Yu, K.-K; Shan, Y.-M.; Li, K.*; Tian J.; Yu, X.-Q.*; Wei, X.* “HClO/ClO– Indicative Interpenetrating Polymer Network Hydrogels as Intelligent Bioactive Materials for Wound Healing” ACS Appl. Bio Mater. 2020, 3, 37-44.
7、Li, K.; Li, L.-L.; Zhou, Q.; Yu, K.-K.; Kim, J. S.*; Yu, X.-Q.* Reaction-based fluorescent probes for SO2 derivatives and their biological applications. Coordin. Chem. Rev. 2019, 388, 310-333.
8、Zhou, Q.; Li, K.*; Li, L.-L.; Yu, K.-K; Zhang, H.; Shi, L.; Chen, H.; Yu, X.-Q.* Combining wittig olefination with photoassisted domino reaction to distinguish 5-formylcytosine from 5-formyluracil. Anal. Chem. 2019, 91, 9366-9370.
9、Zhang H.; Li, K.*; Li, L.-L.; Yu, K.-K; Liu, X.-Y.; Li, M.-Y.; Wang, N.; Liu, Y.-H. Yu, X.-Q.*, Pyridine-Si-xanthene: A novel near-infrared fluorescent platform for biological imaging. Chin. Chem. Lett. 2019, 5, 1063-1066
10、Li, L.-L.; Xu, H.-R.; Li, K,*; Yang, Q.; Pan, S.-L; Yu, X.-Q.* “Mitochondrial G-quadruplex targeting probe with near-infrared fluorescence emission”, Sensor Actuat. B: Chem., 2019, 286, 575-582.
11、Qin, H.-H.; Li, L.-L.; Li, K.*; Yu, X.-Q.* “Novel strategy of constructing fluorescent probe for MAO-B via cascade reaction and its application in imaging MAO-B in human astrocyte”, Chin. Chem. Lett. 2019, 30, 71-74.
12、Shi, L.; Li, K.*; Li, L.-L.; Chen, S. Y.; Li, M.-Y.; Zhou, Q.; Wang, N.; Yu, X.-Q.* “Novel easily available purine-based AIEgens with colour tunability and applications in lipid droplet imaging”, Chem. Sci. 2018, 9, 8969-8974.
13、Zhou, Q.; Li, K.; Liu, Y.-H.; Li, L.-L.; Yu, K.-K; Zhang, H.; Yu, X.-Q.* “Fluorescent Wittig reagent as a novel ratiometric probe for the quantification of 5-formyluracil and its application in cell imaging”, Chem. Commun. 2018, 54, 13722-13725
14、 Li, L.-L.; Li, K.*; Li, M.-Y.; Shi, L.; Liu, Y.-H.; Zhang, H.; Pan, S.-L.; Wang, N.; Zhou, Q.; Yu, X.-Q,* “BODIPY-Based Two-Photon Fluorescent Probe for Real-Time Monitoring of Lysosomal Viscosity with Fluorescence Lifetime Imaging Microscopy”, Anal. Chem. 2018, 90, 5893-5878.
15、Pan, S.-L.; Li, K.*; Li, L.-L.; Li, M.-Y.; Shi, L.; Liu, Y.-H.; Yu, X.-Q.* “A reaction-based ratiometric fluorescent sensor for the detection of Hg(II) ions in both cells and bacteria”, Chem. Commun. 2018, 54, 4955-4958
16、 Yang J.; Li, K.*; Hou, J.-T.; Lu C.-Y.; Li, L.-L.; Yu, K.-K.; Yu, X.-Q.* “A novel coumarin- based water-soluble fluorescent probe for endogenously generated SO2 in living cells”, Sci China Chem, 2017, 60: 793–798.
17、Li, K.#; Hou, J.-T.#; Yang, J.; Yu, X.-Q.* “A tumor-specific and mitochondria-targeted fluorescent probe for real-time sensing of hypochlorite in living cells”, Chem. Commun. 2017, 53, 5539-5541.
18、Hou, J.-T,#; Ren, W.-X.#; Li, K.#; Seo, J.; Sharma, A.; Yu, X.-Q.*; Kim, J.-S.*, “Fluorescent bioimaging of pH: from design to applications” Chem. Soc. Rev. 2017, 46, 2076-2090.
19、Yu, K.-K; Li, K.*; Qin, H.-H.; Zhou, Q.; Qian, C.-H..; Liu, Y.-H.; Yu, X.-Q,*, “Construction of pH-sensitive “Submarine” based on gold nanoparticles with double insurance for intracellular pH mapping, quantifying of whole cells and in vivo applications” ACS Appl. Mater. Interfaces 2016, 8, 22839−22848.
20、Li, L.-L.; Li, K.*; Liu, Y.-H.; Xu, H.-R. Yu,X.-Q.* “Red emission fluorescent probes for visualization of monoamine oxidase in living cells”. Sci. Rep. 2016, 6, 31217.
21、Yang, J.; Li, K.* ; Hou, J.-T.; Li, L.-L.; Lu, C.-Y.; Xie, Y.-M.: Wang, X.; Yu, X.-Q, * “Novel Tumor-Specific and Mitochondria-Targeted near-Infrared-Emission Fluorescent Probe for SO2 Derivatives in Living Cells”, ACS Sens., 2016, 1, 166-172.
22、Liu, Y.; Li, K.*; Xie, K.-X.; Li, L.-L.; Yu, K.-K.; Wang, X.; Yu, X.-Q.* “A water-soluble and fast-response mitochondria-targeted fluorescent probe for colorimetric and ratiometric sensing of endogenously generated SO2 derivatives in living cells”, Chem. Commun. 2016, 52, 3430-3433
